Journal: Ecotoxicology and environmental safety

Article Title: Ameliorative effect of betulinic acid against zearalenone exposure triggers testicular dysfunction and oxidative stress in mice via p38/ERK MAPK inhibition and Nrf2-mediated antioxidant defense activation.

doi: 10.1016/j.ecoenv.2022.113561

Figure Lengend Snippet: Fig. 2. BA protected the aggravation of male reproduction injury in ZEA-induced mice. The morphology of the sperm was photographed using an optical microscope (A). The sperm motility was used to evaluate the male repro duction of mice, including sperm survival rate (B), sperm malformation rate (C), and sperm mortality rate (D). Protein and mRNA levels of ERα in testis were detected by immunoblotting and RT-PCR analysis, respectively, and the pro tein and mRNA levels were normalized to β-actin (E-G). The content of testosterone in serum was detected by ELISA kit (H). The mRNA expression of CLDN11 (I), CDH2 (J), and Vim (K) were measured by RT-PCR. Mean ± SEM, *P < 0.05 and **P < 0.01 represented a significant differ ence compared to the control group, while #P < 0.05 and ##P < 0.01 represented a signifi cant difference compared to the ZEA group.

Article Snippet: Testosterone enzyme linked immunosorbent assay (ELISA) kit (CSB-E05101m) was obtained from Cusabio Biotech Co. Ltd. (Wuhan, China).

Techniques: Microscopy, Western Blot, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Expressing, Control

Journal: Analytical and bioanalytical chemistry

Article Title: Fourier spotting: a novel setup for single-color reflectometry.

doi: 10.1007/s00216-021-03802-w

Figure Lengend Snippet: Fig. 6 Complete sensorgram of a monoclonal anti-testosterone antibodies (CA = 133 nM) with BSA-antigen. After setting the baseline level by PBS (0), association occurs by antibodies-solution (1). Dissociation (2) and detaching of the antibodies (3) lead to a regeneration of the transducer chip where PBS sets the baseline level again (4)

Article Snippet: Source of biological material Testosterone (3-CMO-BSA) Mouse Monoclonal Antibody (BM2076) were obtained from Origene, 32052 Herford, Germany.

Techniques:

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: The ameliorative effect of ellagic acid on di-(2-ethylhexyl) phthalate-induced testicular structural alterations, oxidative stress, inflammation and sperm damages in adult mice

doi: 10.1186/s12958-021-00830-0

Figure Lengend Snippet: The effect of EA on serum testosterone, LH and FSH levels in mice exposed to DEHP. Values are means ± S.D. ( n = 7). * p < 0.05 as compared to control group. # p < 0.05 as compared to DEHP group

Article Snippet: The serum levels of testosterone, LH and FSH were measured using mouse testosterone, LH and FSH ELISA kits (MyBioSource Co, cat num: MBS843463, MBS041300 and MBS2507988, respectively).

Techniques: Control

Journal: PLoS Genetics

Article Title: A Jacob/Nsmf Gene Knockout Results in Hippocampal Dysplasia and Impaired BDNF Signaling in Dendritogenesis

doi: 10.1371/journal.pgen.1005907

Figure Lengend Snippet: (A, B) Testicular morphology in adult wt (A, +/+) and Jacob/Nsmf ko (B, -/-) mice testes stained by hematoxylin and eosin protocol. WT and ko mice showed no differences in appearance of different stages of differentiation from spermatogonia to sperm cells and comparable lumina. (C) Testicular weight of wt (+/+), heterozygous (+/-) and Jacob/Nsmf ko (-/-) mice. (D) Testosteron serum levels were determined in mice of all genotypes by ELISA. Unpaired t-test; *p<0.05. Scale bar in B is 100μm.

Article Snippet: Testosterone plasma levels were determined from serum of adult male wt and Jacob/Nsmf ko mice using the Testosteron ELISA KIT (rat/mouse) (DRG Instruments).

Techniques: Staining, Enzyme-linked Immunosorbent Assay

Journal: PLoS Genetics

Article Title: A Jacob/Nsmf Gene Knockout Results in Hippocampal Dysplasia and Impaired BDNF Signaling in Dendritogenesis

doi: 10.1371/journal.pgen.1005907

Figure Lengend Snippet: (A) Transcript levels of Bdnf exon IV in CA1 and CA3 regions of hippocampus in P10 Jacob/Nsmf ko (-/-) mice exhibit a decrease compared to wt (+/+) mice. (B, C) BDNF-ELISA analysis of CA1 and CA3 tissue samples from P10 and 8–10 weeks old (B) Jacob/Nsmf ko (-/-) and wt (+/+) mice revealed significant lower levels of BDNF only in CA1 region of P10 mice. (C) The expression levels for both regions in mice at the age of 8–10 weeks are similar. (*p < 0.05, **p < 0.01, two-tailed unpaired t-test, values represent mean ± SEM).

Article Snippet: Testosterone plasma levels were determined from serum of adult male wt and Jacob/Nsmf ko mice using the Testosteron ELISA KIT (rat/mouse) (DRG Instruments).

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Two Tailed Test